Primers
| α3 nAChR | |
| Set 1 | |
| Sense | 5′-CTCCAGGTCTGGGGTCTGCGCTG-3′ |
| Antisense | 5′-GCTTTGGTCTTGTCGTCCACCTGG-3′ |
| Set 2 | |
| Sense | 5′-GCCAGTGGCCAGGGCCTCAGAGGC-3′ |
| Antisense | 5′-CCCAGTAGTCCTTGAGGTTCATGG-3′ |
| Set 3 | |
| Sense | 5′-CCATGAACCTCAAGGACT-3′ |
| Antisense | 5′-CACCATGGCAACATATTCC-3′ |
| Set 4 | |
| Sense | 5′-GCCAAAGAGATTCAAGATGATTGGAAGTATGTTGCCATGG-3′ |
| Antisense | 5′-TCTATGTGTCATCTCTGGCCATCAAGGGTTGCAG-3′ |
| α4 nAChR | |
| Set 1 | |
| Sense | 5′-TGCGTGCGCCATGGAGCTAGGGGGC-3′ |
| Antisense | 5′-CGTACGTCCAGGAGCCGAACTTCATG-3′ |
| Set 2 | |
| Sense | 5′-ACGGRMGGGTGCAGTGGA-3′ |
| Antisense | 5′-CTTCTGGCCMGAGCCWG-3′ |
| Set 3 | |
| Sense | 5′-CGGCCCTCCGTGGTCAAGGACAACT-3′ |
| Antisense | 5′-TCCTAGATCATRCCAGCCA-3′ |
| β2 nAChR | |
| Set 1 | |
| Sense | 5′-CGGCTTCAGCACCACGGACAGCGCCCCACC-3′ |
| Antisense | 5′-CCGAGACTCGACCACTGACATGTCGAGTACC-3′ |
| Set 2 | |
| Sense | 5′-ACKGAYACAGAGGAGCGG-3′ |
| Antisense | 5′-GAAGATAAGGTTACGRCACC-3′ |
| Set 3 | |
| Sense | 5′-TCACMTGGAAGCCTGARGA-3′ |
| Antisense | 5′-GGTAGCAGTGGTCGCACA-3′ |
| Set 4 | |
| Sense | 5′-GCGGCGAGAAGATGACGCTGTGCATCTCCG-3′ |
| Antisense | 5′-GGTAGCAGTGGTCGCACA-3′ |