ELISA analysis of cytokine secretion in cultured astrocytes treated with SDF-1α or MIP-1a
Results are expressed as mean ± S.D. of eight separate measurements obtained from different cultures on different days. All values are compared with untreated controls. Cytokine values are expressed as picograms per milliliter.
Condition | TNF-α | IL-1β | IL-6 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 6 h | 24 h | 6 h | 24 h | 6 h | 24 h | ||||
| Untreated control | BDL | BDL | BDL | BDL | BDL | BDL | |||
| SDF-1α | 962.67 ± 65.04*** | 647.25 ± 38.04*** | 611.19 ± 14.66*** | 420.88 ± 20.84*** | BDL | BDL | |||
| with CXCR4 IAb | BDL | BDL | BDL | BDL | BDL | BDL | |||
| with CCR5 IAb | 997.67 ± 42.17*** | 710.17 ± 25.20*** | 592.75 ± 38.55*** | 405.88 ± 14.25*** | BDL | BDL | |||
| MIP-1α | 806.83 ± 51.33*** | 521.83 ± 31.92*** | 593.69 ± 8.25*** | 381.19 ± 24.27*** | 405.88 ± 59.53*** | 304.43 ± 41.55*** | |||
| with CXCR4 IAb | 916.00 ± 23.51*** | 588.92 ± 15.95*** | 609.00 ± 27.72*** | 420.88 ± 24.85*** | 395.88 ± 43.67*** | 314.43 ± 38.25*** | |||
| with CCR5 IAb | BDL | BDL | BDL | BDL | BDL | BDL | |||
IAb, inhibitory antibody; BDL, below the detection limit of the assay.
↵ *** p < 0.001.