TABLE 1

EC50 values and maximal (Max) responses of cAMP formation in control and advAC6 (10 pfu/cell)-treated cells

Values are presented as mean ± S.E.M. (n ≥ 3).


Drug

EC50

Max Response

Max Response Ratio
Control
advAC6
Control
advAC6
μM pmol/106 cells
Forskolin 10.1 ± 0.3 4.4 ± 0.2 28.0 ± 2.1 213 ± 17 7.6
Histamine 13.6 ± 0.8 11.2 ± 0.4 5.3 ± 0.7 12.0 ± 1.2 2.3
Isoproterenol 0.32 ± 0.06 0.7 ± 0.1 8.1 ± 1.0 18.2 ± 1.8 2.2
SKF-38393 1.5 ± 0.1 3.7 ± 0.2
PGE2 high affinity 0.002 ± 0.0001 0.01 ± 0.001 16.2 ± 1.4 142 ± 12 8.9
PGE2 low affinity 0.5 ± 0.04 16.0 ± 0.3
Beraprost 0.1 ± 0.02 4.4 ± 0.2 5.8 ± 0.7 46.0 ± 6.4 7.9
cPGI2 5.9 ± 0.1 12.5 ± 0.4 11.8 ± 0.8 84.3 ± 1.1 7.1
11-Deoxy PGE1 0.08 ± 0.01 0.13 ± 0.01 5.0 ± 0.4 11.0 ± 0.8 2.2
BW245C
5.0 ± 0.3
2.9 ± 0.1
4.5 ± 0.4
6.0 ± 0.3
1.3