TABLE 1

Effect of different steroids on the OATP2B1-mediated E3S uptake OATP2B1-transfected MDCKII cells were incubated with [3H]E3S (1 μCi/ml; 1 μM) for 5 min in the presence of indicated compounds (n = 3).


Substance

Concentration

% of Control (Mean ± S.D.)
μM
Control 100
Aldosterone 100 110 ± 11
Aldosterone 10 129 ± 15
Androsterone 100 145 ± 20*
Androsterone 10 105 ± 9
Cortisone 100 107 ± 13
Cortisone 10 125 ± 15
Estrone 100 68 ± 8*
Estrone 10 87 ± 7
Estriol 100 43 ± 10*
Estriol 10 96 ± 5
β-Estradiol 10 35 ± 4*
Hydrocortisol 100 83 ± 15
Hydrocortisol 10 92 ± 7
Hydroxyprogesterone 10 168 ± 12*
Mifepristone 100 7 ± 1*
Mifepristone 10 26 ± 4*
Pregnenolone 50 129 ± 4
Pregnenolone 10 171 ± 27*
Progesterone 100 66 ± 5
Progesterone 10 186 ± 14*
Testosterone 100 27 ± 4*
Testosterone 10 84 ± 8
Conjugates
Estrone-3-sulfate 10 43 ± 5*
Estradiol-glucuronide 10 106 ± 3
DHEAS 10 70 ± 2*
Pregnenolone sulfate 10 28 ± 7*
Pregnenolone acetate
10
83 ± 15
  • * Significant vs. control for P < 0.05.