TABLE 2

Effect of different steroids on the OATP2B1-mediated DHEAS uptake OATP2B1-transfected MDCKII cells were incubated with [3H]DHEAS (1 μCi/ml) for 10 min in the presence of indicated compounds (n = 3).


Substance

Concentration

% of Control (mean ± S.D.)
μM
Control 100 ± 3
Androsterone 100 121 ± 5
Androsterone 10 159 ± 27*
Estrone 100 283 ± 43*
Estrone 10 288 ± 57*
Estriol 100 78 ± 13
Estriol 10 108 ± 8
β-Estradiol 10 158 ± 15*
Hydrocortisol 100 158 ± 35
Hydrocortisol 10 93 ± 11
Hydroxyprogesterone 100 241 ± 6*
Hydroxyprogesterone 10 209 ± 13*
Mifepristone 100 55 ± 15*
Mifepristone 10 69 ± 10*
Pregnenolone 50 179 ± 14*
Pregnenolone 10 184 ± 12*
Progesterone 100 303 ± 36*
Progesterone 10 346 ± 3*
Testosterone 100 55 ± 4*
Testosterone 10 75 ± 6*
Conjugates
Estrone-3-sulfate 100 64 ± 7*
Estrone-3-sulfate 10 92 ± 10
Estradiol-glucuronide 10 95 ± 13
Pregnenolone sulfate
100
43 ± 7*
  • * Significant vs. control for P < 0.05.