TABLE 3

Uptake of [3H]E3S (20 nM, 1 μCi/ml), [3H]DHEAS (17 nM, 1 μCi/ml), [3H]progesterone (20 nM, 1 μCi/ml), and [3H]testosterone (20 nM, 1 μCi/ml) into OATP2B1-transfected and -nontransfected MDCKII cells Cells were incubated for 15 min with the respective substance (n = 3).




MDCKII Mean ± S.D.

OATP2B1 Mean ± S.D.

OATP2B1-Specific
fmol / min / mg
E3S 11.1 ± 1.1 301 ± 33* 290
DHEAS 11.4 ± 1.9 36.3 ± 3.5* 24.9
Progesterone 488 ± 20 354 ± 44* −135
Testosterone
67.3 ± 5.4
56.1 ± 9.9
−11.2
  • * Significant vs. MDCKII for P < 0.05.