TABLE 1

CREB phosphorylation in response to cAMP-elevating agents in rat cardiac fibroblasts

Cells were treated with the indicated agent for the given amount of time before levels of pCREB were measured by immunoblot analysis (see Materials and Methods). Immunoreactive bands were quantified and then normalized to the levels of total CREB immunoreactivity. The data are expressed as the mean ± S.E.M. of four to five experiments.




Basal

5 min

20 min

30 min

1 h

3 h

24 h
Fsk 10 μM 12.4 ± 0.1 17.9 ± 0.1* 15.5 ± 0.5* 18.2 ± 2.6* 17.5 ± 0.9* 11.7 ± 2.0 6.8 ± 0.8*
Iso 1 μM 9.3 ± 1.4 14.6 ± 1.1* 14.1 ± 1.0* 17.5 ± 2.2* 15.8 ± 1.3* 15.8 ± 2.2 12.8 ± 2.1
PGE2 1 μM
13.8 ± 0.6
19.2 ± 2.0*
14.0 ± 0.6
13.1 ± 0.3
13.7 ± 0.4
13.0 ± 0.7
13.0 ± 1.3
  • * p < 0.05 compared with basal by ANOVA.