TABLE 2

IL-1β-induced COX-2 and IL-8 mRNA decay: effect of dexamethasone and RU24858

Using the mRNA decay data presented in Fig. 6., the slope for each set of experimental values was calculated by linear regression analysis (GraphPad Prism) using values for t = 0, 0.25, 0.5, and 1 h. In each case, this provides an initial slope in which active mRNA degradation appeared to be occurring. Data (n = 7-8) for each initial slope are presented as means ± S.E. Significance or nonsignificance (N.S.) between these values is indicated.


Treatment

COX-2


IL-8

Initial Slope
P

Initial Slope
P

mean ± S.E. mean ± S.E.
IL-1β −9.0 ± 5.3 * N.S.
−38.3 ± 5.0 *** *
IL-1β + dexamethasone −32.9 ± 4.9 −72.6 ± 2.7
IL-1β + RU24858
−22.4 ± 4.9
N.S.
−55.9 ± 3.4
*
  • *P < 0.05; ***P < 0.001.