Primers used for plasmid construction
Listed primers used for PCR amplification of rat Mrp2 5′UTR cDNAs are shown, with the cloning sites underlined.
Name | Sequence (5′-3′) | Cloning Site |
|---|---|---|
| TRF1 | 5′-ggaagcttatgtctgctcactggga-3′ | HindIII |
| TRF2 | 5′-ggaagcttattaagtcgtcaggatga-3′ | HindIII |
| TRF3 | 5′-tcaaagcttaggcctttaactgggctg-3′ | HindIII |
| TRF4 | 5′-ggaaagcttacggtgcactttaacatctg-3′ | HindIII |
| TRF5 | 5′-ggaagcttagaggaaaaagtaaaggag-3′ | HindIII |
| CONR | 5′-accccatggtaatgctctcctcgcgc-3′ | NcoI |
| T7R1 | 5′-ggggatccgaatgctctcctcgcgc-3′ | BamHI |
| T7R2 | 5′-tttggatccaatgctctcctcgcg-3′ | BamHI |
| TRRR | 5′-ggggatccaatgctctcctcgcgc-3′ | BamHI |