TABLE 1

Binding affinities of flunitrazepam and zolpidem for α1β2γ2s WT and α1β2ϰ receptors

Values shown are the mean ± S.E.M. for n number of independent experiments. K_I is the equilibrium dissociation constant (apparent affinity) of the unlabeled ligand. K_I values for flunitrazepam were determined by [³H]flunitrazepam saturation and competition binding experiments. K_I values for zolpidem were determined by the displacement of [³H]flunitrazepam. Statistical differences between WT and chimera logK_I values were determined using one-way analysis of variance with Dunnett's post test.