The summary of single-channel kinetic analysis from the wild-type receptor under control conditions and in the presence of combinations of ethanol and 3α5αP in the absence and presence of steroid antagonist, 17-PA
The mean durations (OT1-3, CT1, CT2, and CTβ) and relative contributions (fraction OT1-3, fraction CT1, CT2, and CTβ) for the three open and closed time components are shown. All data were obtained in the presence of 50 μM GABA to activate the channel. The control data (no steroid, no ethanol) are from Akk et al. (2005). Statistical analysis was carried out using ANOVA with Bonferroni correction (Systat 7.0; Systat Software, Inc., Point Richmond, CA). For 3α5αP, the significance level applies to comparison to no steroid/no ethanol (control) condition. For 3α5αP + 17-PA, the significance levels apply to comparison with control condition and to 3α5αP alone, respectively.
Steroid | Ethanol | OT1 | Fraction OT1 | OT2 | Fraction OT2 | OT3 | Fraction OT3 | CT1 | Fraction CT1 | CT2 | Fraction CT2 | CTβ | Fraction CTβ | n |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ms | ms | ms | ms | ms | ms | |||||||||
| None | None | 0.28 ± 0.07 | 0.24 ± 0.04 | 3.1 ± 0.8 | 0.58 ± 0.10 | 7.6 ± 3.0 | 0.19 ± 0.13 | 0.17 ± 0.02 | 0.57 ± 0.06 | 1.7 ± 0.7 | 0.14 ± 0.05 | 13.5 ± 5.3 | 0.29 ± 0.02 | 8 |
| 10 nM 3α5αP | 0.1% | 0.35 ± 0.04 | 0.32 ± 0.05 | 3.1 ± 0.8 | 0.39 ± 0.02 | 18.0 ± 4.5** | 0.29 ± 0.05† | 0.17 ± 0.02 | 0.58 ± 0.01 | 1.4 ± 0.2 | 0.26 ± 0.02 | 13.4 ± 1.7 | 0.16 ± 0.02*** | 3 |
| 10 nM 3α5αP + 10 μM 17-PA | 0.1% | 0.28 ± 0.09 | 0.31 ± 0.07 | 2.9 ± 1.0 | 0.39 ± 0.17 | 9.7 ± 3.5†,** | 0.30 ± 0.17†,† | 0.17 ± 0.02 | 0.56 ± 0.10 | 1.9 ± 0.5 | 0.25 ± 0.12 | 13.4 ± 5.0 | 0.19 ± 0.06***,† | 7 |