TABLE 1

ChIP analysis of C/EBP and NFI binding to CYP2A13 and Cyp1a2 promoters in the olfactory mucosa of the CYP2A13-transgenic mice

Tissues were pooled from three male, 2-month-old homozygous mice. Equal amounts of chromatin were used for each immunoprecipitation experiment. Each immunoprecipitated sample was analyzed for the abundance of CYP2A13 and Cyp1a2 promoter fragments using real-time PCR relative to the amounts of each promoter detected in samples precipitated by the control IgG. The values presented (means ± S.D., n = 3) are in arbitrary units. The results shown are typical of three independent experiments performed.

Antibody
Relative Abundance of Promoter Fragments
CYP2A13/Cyp1a2 Ratio
p Valuea
CYP2A13
Cyp1a2
Normal IgG 100 ± 17 100 ± 16 1.00
Anti-C/EBPα 131 ± 12 114 ± 11 1.15 0.17
Anti-C/EBPβ 93 ± 15 108 ± 7 0.86 0.17
Anti-C/EBPδ 174 ± 3b 121 ± 18 1.43 0.008
Anti-NFI
125 ± 21
259 ± 25b
0.48
0.002

  • ↵ a For differences between the relative amounts of CYP2A13 and Cyp1a2 in each sample (Student's t test).

  • ↵ b Significantly higher than in samples incubated with normal IgG (P < 0.01).