TABLE 1

[3H]Spiperone binds with similar and high affinity to various D2L receptor-G protein fusions

Individual D2L-G protein fusions were expressed transiently in HEK293 cells. Saturation [3H]spiperone ligand binding studies were performed on membrane preparations as detailed under Materials and Methods. Data represent means (±S.E.M.) of studies performed on membranes prepared from three individual transfections.


Receptor:G Protein Fusion

Kd

Bmax
nM fmol/mg
D2L-Gαi1 0.049 (0.003) 1500 (49)
D2L-Gαi2 0.051 (0.003) 1415 (24)
D2L-Gαi3 0.057 (0.002) 1976 (14)
D2L-Gαo1
0.057 (0.006)
1508 (43)