TABLE 1

Radioligand binding properties of wild-type and mutant cell lines.

The Kd and Bmax values were determined from saturation binding experiments using [3H]CP55,940 or [3H]WIN55,212-2 on HEK 293 cell membrane preparations stably transfected with wild-type or mutant hCB1 receptor. Data represent the mean and corresponding 95% confidence limits of at least three independent experiments performed in triplicate. No significant difference was observed between the wild-type and mutant binding properties as determined by two-tailed Student t test.


Radioligand & Cell Line

Kd

Bmax
nM pmol / mg
[3H]CP55,940
    WT 2.0 (0.7-3.2) 1.03 (0.8-1.3)
    S7.39A N.B.
    S2.60A 4.2 (1.5-6.8) 3.3 (2.3-4.5)
[3H]WIN55,212-2
    WT 4.7 (1.8-7.5) 1.2 (1.0-1.6)
    S7.39A
6.0 (5.4-6.5)
1.9 (0.5-2.4)
  • N.B., no specific binding observed.