TABLE 1

Relative Bmax and Kd values obtained from [3H]GR65630 saturation binding to membranes of HEK293 cells transiently expressing either homopentameric 5-HT3A receptors or heteromeric receptors of 5-HT3A with one of the novel subunits




5-HT3A (Control)

5-HT3A/C

5-HT3A/D

5-HT3A/E

5-HT3A

5-HT3A/Ea
Bmax (% of control) 100.00 ± 9.30 96.58 ± 6.57 143.61 ± 10.14* 147.44 ± 11.32* 100.00 ± 2.63 81.02 ± 4.24**
Kd (nM)
0.39 ± 0.03
0.29 ± 0.03
0.46 ± 0.06
0.42 ± 0.05
0.30 ± 0.04
0.35 ± 0.09
  • Experiments were performed in two separate groups. Bmax values are expressed as the percentage fraction of the Bmax of the homopentameric 5-HT3A receptor (percentage of control). Significant differences (one-way ANOVA followed by Dunnett's post test) compared with homopentameric 5-HT3A receptors; *p < 0.05, and **p < 0.01.