TABLE 1

Oligonucleotides used for the construction of the UGT1A3 and UGT1A4 mutants at residues 36 and 40 Mutagenized positions are underlined. Accession numbers for UGT1A3 and UGT1A4 are AY435138.1 and AY435139.1, respectively.


Mutant

Primers
UGT1A3(I36T) 5′-GTGTTGGTGGTGCCCACTGATGGCAGCCACTGG-3′
UGT1A3(H40P) 5′-CCCATTGATGGCAGCCCCTGGCTCAGCATGCGG-3′
UGT1A4(T36I) 5′-GGTGTTGGTGGTGCCCATTGATGGCAGCCCCTG-3′
UGT1A4(P40H)
5′-CCCACTGATGGCAGCCACTGGCTCAGCATGCGG-3′