TABLE 1

Competition of CerN-γ subunits with YN-γ2 for dimerization with CC-β5 in live HEK-293 cells Values represent the mean ± S.E. from three experiments.

γ subunit
IC50a
CerN-γ Expressionb
Normalized IC50c
μg of CerN-γ per μg (× 10–5) × 10–5
γ1 3.10 ± 1.08 1.38 ± 0.23 4.27 ± 1.65
γ2 0.15 ± 0.02 4.44 ± 0.77 0.66 ± 0.14
γ5 2.15 ± 0.59 4.39 ± 0.17 9.43 ± 2.64
γ7 0.40 ± 0.06 6.78 ± 0.58 2.68 ± 0.49
γ10 2.66 ± 0.48 3.27 ± 0.07 8.71 ± 1.57
γ11 1.94 ± 0.54 7.88 ± 0.58 15.27 ± 4.41
γ12
1.60 ± 0.31
5.62 ± 0.46
9.01 ± 1.88

  • ↵ a Defined as micrograms of CerN-γ plasmid that produced a 50% decrease in the intensity of CC-β5YN-γ2, calculated from the data in Fig. 3D

  • ↵ b Calculated from linear fits of CerN-γ expression per microgram plasmid. Expression levels were determined as described in the legend to Fig. 3E

  • ↵ c Defined as IC50 × CerN-γ expression per microgram of plasmid