Activity | Wild Type | 10ΔC | 10ΔC-F483C |
---|---|---|---|
[14C]TEA uptake | |||
Vmax (pmol · oocyte−1 · h−1) | 503 ± 73 (4) | 560 ± 95 (3) | 385 ± 68 (4) |
Km (μM) | 52 ± 21 (11) | 67 ± 8 (3) | 143 ± 27 (3)***,††† |
[3H]MPP uptake | |||
Vmax (pmol · oocyte−1 · h−1) | 122 ± 30 (5) | 133 ± 23 (3) | 57 ± 9 (4) |
Km (μM) | 3.9 ± 1.1 (3) | 2.5 ± 1.0 (2) | 3.0 ± 0.7 (3) |
TEA induced current | |||
Imax (nA) | 6.4 ± 2.3 (5)a | 15.3 ± 1.7 (4)* | 9.2 ± 4.1 (3) |
K0.5 (μM) | 30 ± 3 (5)a | 54 ± 5 (4) | 118 ± 8 (3)**,† |
Choline-induced current | |||
Imax (nA) | 18 ± 13 (46)a | 43 ± 11 (9)*,a | 25 ± 3.4 (10) |
K0.5 (μM) | 290 ± 180 (6)a | 1410 ± 240 (3)***,a | 1300 ± 160 (10)*** |