Primers for amplification of human and mouse mRNA
Organism and Pair No. | Target Gene | GenBank Accession No. | Oligonucleotides (5′-3′) | Product Size | ||
|---|---|---|---|---|---|---|
| Forward | Reverse | |||||
| bp | ||||||
| Homo sapiens | ||||||
| 1 | β-Actin Ndrg1 | NM_001101 | CCCGCCGCCAGCTCACCATGG | AAGGTCTCAAACATGATCTGGGTC | 397 | |
| 2 | TfR1 | NM_006096 | CCCTCGCGTTAGGCAGGTGA | AGGGGTACATGTACCCTGCG | 370 | |
| 3 | NM_003234 | GCTCGGCAAGTAGATGGC | TTGATGGTGCTGGTGAAG | 359 | ||
| Mus musculus | ||||||
| 4 | β-Actin | NM_007393 | CCCGCCACCAGTTCGCCATGG | AAGGTCTCAAACATGATCTGGGTC | 397 | |
| 5 | HIF-1α | NM_010431 | CTGGATGCCGGTGGTCTAGACAGT | CGAGAAGAAAAAGATGAGTTCTGAACGTCG | 217 | |
| 6 | Ndrg1 | NM_008681 | TGCTTGCTCATTAGGTGTGTGATAGC | CCATCCTGAGATCTTAGAGGCAGC | 581 | |
| 7 | TfR1 | NM_011638 | TCCCGAGGGTTATGTGGC | GGCGGAAACTGAGTATGATTGA | 324 | |
| 8 |
VEGF1
| NM_009505 | CCATGCCAAGTGGTCCCAG | GTCTTTCTTTGGTCTGCATTCACAT | 346 | |