Primers and fluorescent probes
CRLR |
|
| Forward | 5′-CATGGACAAATTATACCCAGTGT-3′ |
| Probe | 5′- (FAM) CACCCACGAGAAAGTGAAGACTGCA (TAMRA)-3′ |
| Reverse | 5′-TCCAATTATGGTCAGGTAAAACAA-3′ |
| RAMP1 | |
| Forward | 5′-GGAGACGCTGTGGTGTGAC-3′ |
| Probe | 5′- (FAM) ACCATCAGGAGCTACAGGGAGCTGG (TAMRA)-3′ |
| Reverse | 5′-CATGTGCCAGGTGCAGTC-3′ |
| RAMP2 | |
| Forward | 5′-CTCAGCCTCTTCCCACCAC-3′ |
| Probe | 5′- (FAM) CACACCAGGGTCAGAAGGGGG (TAMRA)-3′ |
| Reverse | 5′-TTCCAGCAAAATTGGACAGC-3′ |
| RAMP3 | |
| Forward | 5′-AAGGTGGACGTCTGGAAGTG-3′ |
| Probe | 5′- (FAM) CCTGTCCGAGTTCATCGTGTACTATGAGA (TAMRA)-3′ |
| Reverse | 5′-ATCTCGGTGCAGTTGGTGA-3′ |
| β-actin | |
| Forward | 5′-CGTCTTCCCCTCCATCGT-3′ |
| Probe | 5′- (FAM) AGGCACCAGGGCGTGATGGT (TAMRA)-3′ |
| Reverse | 5′-GGAATCCTTCTGACCCATGC-3′ |
FAM, 5-carboxyfluorescein; TAMRA, 5-carboxytetramethylrhodamine.