TABLE 1

Oligonucleotide primer sequences

Primers Sequence GenBank Accession Numbers
SUR1 NM_011510
First-round
PCR
Forward 5′-CGAGAGTCCCTTCAATAAGCAA-3′
Reverse 5′-AATGCTCAAAGAGCTGGCACTC-3′
Nested PCR
Forward; 5′-CCCTCTACCAGCACACCAA-3′
Reverse 5′-CAGTCTGCATGAGGCAGGTA-3′
SUR2 D86037 (SUR2A) D86038 (SUR2B)
First-round
PCR
Forward 5′-TGGACAGAGACAGCTGTTCTG-3′
Reverse 5′-AGGCAAACACTCCATCTTCCTG-3′
Nested PCR
Forward 5′-AGCCACTGCTTCCATCGACA-3′
Reverse 5′-CCTCTCTTCATCACAATAACCAG-3′
β-actin NM_007393
First-round
PCR
Forward 5′-CTACGAGGGCTATGCTCTCC-3′
Reverse 5′-CTTCTGCATCCTGTCAGCAA-3′
Nested PCR
Forward 5′-GCTACAGCTTCACCACCACA-3′
Reverse 5′-AAGGAAGGCTGGAAAAGAGC-3′
Pecam-1 NM_001032378
First-round
PCR
Forward 5′-AGAGACGGTCTTGTCGCAGT-3′
Reverse 5′-AATGGCAATTATCCGCTCTG-3′
Nested PCR
Forward 5′-TGCTCTCGAAGCCCAGTATT-3′
Reverse 5′-TGTGAATGTTGCTGGGTCAT-3′
Myh11 NM_013607
First-round
PCR
Forward 5′-AGCCGGAAAGACAGAGAACA-3′
Reverse 5′-ACTTTCTGAGCCGCTGTGTT-3′
Nested PCR
Forward 5′-GACAACTCCTCTCGCTTTGG-3′
Reverse 5′-GCTCTCCAAAAGCAGGTCAC-3′
PGP9.5 NM_011670
First-round
PCR
Forward 5′-CGAAGATAGAGCCAAGTGTT-3′
Reverse 5′-GAAAGTGCTCAGCCTGGTGT-3′
Nested PCR
Forward 5′-GAATGCCCTTTCCAGTGAAC-3′
Reverse 5′-GCTAAAGCTGCAAACCAAGG-3′
FABP4 NM_024406
First-round
PCR
Forward 5′-CAGCCTTTCTCACCTGGAAG-3′
Reverse 5′-TGGCTCATGCCCTTTCATAA-3′
Nested PCR
Forward 5′-CATCAGCGTAAATGGGGATT-3′
Reverse 5′-TCGACTTTCCATCCCACTTC-3′