Not Pretreated | 30 min | 60 min | 120 min | 240 min | Pretreatment | ||
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pEC50 | |||||||
DAMGO | 8.03 ± 0.16 (5) | 7.23 ± 0.10 (5)* | 7.32 ± 0.06 (5)* | 7.07 ± 0.11 (5)* | 7.28 ± 0.10 (5)* | DAMGO 1 μM | |
Morphine | 7.64 ± 0.11 (5) | 7.80 ± 0.08 (5) | 7.71 ± 0.11 (5) | 7.78 ± 0.14 (5) | 7.81 ± 0.18 (5) | Morphine 1 μM | |
DAMGO | 8.00 ± 0.15 (5) | 8.13 ± 0.24 (5) | 7.92 ± 0.22 (5) | 7.96 ± 0.17 (5) | 8.03 ± 0.20 (5) | 5-HT 1 μM | |
DAMGO | 7.64 ± 0.11 (5) | 8.07 ± 0.13 (5) | 8.20 ± 0.08 (5)* | 8.15 ± 0.07 (5)* | 8.14 ± 0.11 (5)* | 5-HT 1 μM | |
DAMGO | 8.00 ± 0.02 (3) | 6.98 ± 0.14 (3)* | 7.15 ± 0.08 (3)* | 6.92 ± 0.31 (3)* | 6.99 ± 0.20 (3)* | DAMGO 1 μM | |
5-HT 1 μM | |||||||
Morphine | 7.86 ± 0.14 (3) | 7.25 ± 0.10 (3)* | 7.24 ± 0.10 (3)* | 7.20 ± 0.18 (3)* | 7.22 ± 0.16 (3)* | Morphine 1 μM | |
5-HT 1 μM | |||||||
% Not pretreated Emax | |||||||
DAMGO | 100 (5) | 87.30 ± 5.53 (5) | 89.99 ± 4.00 (5) | 86.98 ± 3.90 (5) | 85.57 ± 3.05 (5) | DAMGO 1 μM | |
Morphine | 100 (5) | 102.26 ± 2.06 (5) | 100.73 ± 1.84 (5) | 96.03 ± 5.66 (5) | 98.15 ± 3.80 (5) | Morphine 1 μM | |
DAMGO | 100 (5) | 94.77 ± 3.42 (5) | 102.89 ± 3.15 (5) | 93.44 ± 3.37 (5) | 92.31 ± 4.71 (5) | 5-HT 1 μM | |
Morphine | 100 (5) | 101.05 ± 2.43 (5) | 100.09 ± 6.72 (5) | 102.20 ± 7.69 (5) | 103.99 ± 5.71 (5) | 5-HT 1 μM | |
DAMGO | 100 (3) | 96.27 ± 5.44 (3) | 90.88 ± 5.30 (3) | 74.73 ± 4.41 (3)* | 78.51 ± 1.74 (3)* | DAMGO 1 μM | |
5-HT 1 μM | |||||||
Morphine | 100 (3)* | 79.37 ± 1.26 (3)* | 70.59 ± 3.01 (3)* | 64.10 ± 3.08 (3)* | 72.40 ± 3.15 (3)* | Morphine 1 μM | |
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| 5-HT 1 μM |
↵* Different from not treated. P < 0.05 by Dunnett's post hoc test analysis of variance.