Stimulation of [35S]GTPγS binding by FFA2 agonists in the presence of different concentrations of allosteric ligands on FFA2 receptor
The logKB values and cooperativity factors of the ligands are calculated by fitting the data to allosteric global pEC50 equation (see Materials and Methods). Data represented is mean ± S.E.M. from two to three independent experiments performed in duplicate.
Ligands | Log KB | Log α (Cooperativity Factor) |
|---|---|---|
| Acetate + phenylacetamide 1 | −6.38 ± 0.73 | 0.72 ± 0.03 |
| Acetate + phenylacetamide 2 | −6.2 ± 0.39 | 1.0 ± 0.133 |
| Propionate + phenylacetamide 1 | −6.0 ± 0.66 | 1.1 ± 0.27 |
| Propionate + phenylacetamide 2 | −6.67 ± 0.90 | 0.6 ± 0.07 |
| Phenylacetamide 1 + acetate | −3.5 ± 0.37 | 1.14 ± 0.3 |
| Phenylacetamide 2 + acetate | −3.0 ± 0.05 | 1.13 ± 0.08 |
| Phenylacetamide 1 + propionate | −3.5 ± 0.14 | 0.97 ± 0.02 |
| Phenylacetamide 2 + propionate | −3.3 ± 0.57 | 1.2 ± 0.08 |