TABLE 2
Binding characteristics of the wild type and A4 mutant dopamine D2 receptors
Affinity values for [3H]spiperone (Kd) were determined by saturation analyses. The apparent affinity values for dopamine were determined by inhibition of the binding of[3H]spiperone, followed by nonlinear regression analysis of the competition curves. Two-site fits generated values for binding sites with high affinity (Kihigh) and low affinity (Kilow) for dopamine. Each value represents the mean ± S.E. of three or more independent experiments.