Expression of FGF and FGFR mRNAs in primary NSCLC tumors
Primary lung tumor total RNA obtained through the Tissue Procurement Core of the UCHSC Lung SPORE was reverse-transcribed and submitted to quantitative RT-PCR with primers (see Table 1) specific for FGF2, FGF9, FGFR1, or FGFR2. The values are normalized to GAPDH mRNA and presented as mean relative expression and S.E.M. The status of the FGFR IIIb/IIIc splice variants for FGFR1 and FGFR2 was determined as described in the legend to Fig. 4. FGFR1 IIIc was the sole FGFR1 isoform detected in the primary tumor mRNA samples.
Tumor ID | Relative Expression | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| FGF2 | FGF9 | FGFR1 | FGFR2 | FGFR2 isoform | |||||
| 11898 | 0.06 ± 0.001 | 0.04 ± 0.018 | 0.12 ± 0.027 | 0.29 ± 0.037 | FGFR2b | ||||
| 18187 | 1.12 ± 0.24 | 0.00 ± 0.000 | 0.32 ± 0.073 | 0.56 ± 0.038 | FGFR2c | ||||
| 16219 | 0.14 ± 0.000 | 0.46 ± 0.089 | 0.39 ± 0.115 | 0.07 ± 0.014 | |||||
| 21023 | 1.00 ± 0.000 | 0.00 ± 0.001 | 0.49 ± 0.051 | 1.00 ± 0.000 | FGFR2c | ||||
| 16895 | 0.13 ± 0.010 | 0.13 ± 0.055 | 0.18 ± 0.035 | 0.04 ± 0.001 | |||||
| 21236 | 0.06 ± 0.025 | 1.00 ± 0.000 | 0.31 ± 0.045 | 0.51 ± 0.127 | FGFR2b and c | ||||
| 21211 | 0.05 ± 0.002 | 0.01 ± 0.003 | 1.00 ± 0.000 | 0.03 ± 0.010 | |||||
| 21460 | 0.04 ± 0.027 | 0.01 ± 0.005 | 0.10 ± 0.040 | 0.03 ± 0.004 | |||||
| 21450 | 0.16 ± 0.018 | 0.03 ± 0.011 | 0.40 ± 0.030 | 0.46 ± 0.088 | FGFR2b and c | ||||