TABLE 1

V½ values of various chimeras and mutants in the absence or presence of retigabine.


Construct

V½

V½ (10 μM RGB)

ΔV½ (10 μM RGB)
mV mV mV
KCNQ3 −50 ± 0.9 −89.4 ± 1.9 −39.4 ± 2.1
KCNQ3(TD;Q1) −28.2 ± 1.4 −73.7 ± 0.8 −45.5 ± 1.6
KCNQ3(P;Q1) −36.8 ± 1.2 −54.2 ± 1.2 −17.4 ± 1.7
KCNQ3(P;Q1)VV.VT-LI.LA −44.1 ± 1.3 −78.9 ± 1.3 −34.8 ± 1.8
KCNQ3(P;Q1)V.Q-T.K −37.3 ± 0.6 −37.8 ± 1.3 −0.5 ± 1.4
KCNQ3(P;Q1)V311L −37.7 ± 0.6 −52.3 ± 0.8 −14.6 ± 1.0
KCNQ3(P;Q1)V312I −47.7 ± 1.2 −55.8 ± 1.0 −8.1 ± 1.6
KCNQ3(P;Q1)V314L −56.1 ± 1.2 −80.6 ± 2.2 −24.5 ± 2.5
KCNQ3(P;Q1)T315A −39.5 ± 1.4 −43.9 ± 1.0 −4.4 ± 1.7
KCNQ3(TD;Q1)L314V −32.9 ± 0.5 −40.5 ± 1.0 −7.6 ± 1.1
KCNQ1-L266W+V310L −15.6 ± 0.9 −16.3 ± 1.4 −0.7 ± 1.7
KCNQ1-L266W+V310L-sidQ3/KCNQ2 −27.8 ± 0.5 −46.0 ± 1.3 −18.2 ± 1.4
KCNQ3(TD;Q1)L338V −24.9 ± 1.5 −43.3 ± 1.2 −18.4 ± 1.5
KCNQ1-L266W+V310L + V334LsidQ33 21.8 ± 0.9 9.7 ± 1.9 −12.1 ± 1.1
KCNQ1-L266W+V310L + V334LsidQ3/KCNQ2
−17.5 ± 0.6
−23.7 ± 0.5
−6.2 ± 1.1