Agonist | Control | 4-DAMP Mustard | Furchgott Analysis | ||||||||||||
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EC50 (log molar) | Emax | Hill Slope | EC50 (log molar) | Emax | Hill Slope | Kobs (log molar) | ϵ | q | |||||||
μM | % | μM | % | μM | |||||||||||
Oxotremorine-M | 0.30 (−6.53 ± 0.07) | 123 ± 5.7 | 1.07 ± 0.16 | 2.04 (−5.69 ± 0.12) | 67.3 ± 6.9 | 1.07 ± 0.19 | 1.84 (−5.73 ± 0.14) | 2.89 (0.46 ± 0.26) | 0.18 ± 0.053 | ||||||
Oxotremorine | 0.39 (−6.41 ± 0.17) | 98.4 ± 10.5 | 1.02 ± 0.32 | 0.54 (−6.27 ± 0.13) | 33.0 ± 3.7 | 2.62 ± 2.3 | 0.68 (−6.17 ± 0.15) | 0.74 ± 1.39 (−0.13 ± 0.20) | 0.24 ± 0.06 | ||||||
Carbachol | 1.21 (−5.94 ± 0.06) | 99.9 ± 3.5 | 1.11 ± 0.14 | 7.32 (−5.14 ± 0.17) | 44.4 ± 4.8 | 0.89 ± 0.21 | 3.24 (−5.49 ± 0.15) | 1.0 | 0.29 ± 0.06 | ||||||
S-Aceclidine | 3.07 (−5.51 ± 0.16) | 109.8 ± 11.5 | 1.16 ± 0.41 | 7.71 (−5.11 ± 0.12) | 54.8 ± 5.3 | 1.16 ± 0.29 | 2.92 (−5.53 ± 0.25) | 0.78 (−0.11 ± 0.20) | 0.46 ± 0.12 | ||||||
McN-A-343 | 2.58 (−5.59 ± 0.12) | 17.9 ± 1.4 | 1.52 ± 0.52 | 1.66a (−5.78 ± 0.61) | 0.093a (−1.03 ± 0.06) | ||||||||||
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| 2.86b (−5.54 ± 0.35) | 0.084b (−1.07 ± 0.06) |
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↵ a The affinity and intrinsic efficacy of McN-A-343 was determined through the RAi analysis using the null method. The log mean ± S.E.M. values are shown in parentheses.
↵ b The affinity and intrinsic efficacy of McN-A-343 was determined through the RAi analysis using the operational method. The log mean ± S.E.M. values are shown in parentheses.