Analytical data for compounds 1-13
Calculated indicates the theoretical monoisotopic MH+ value. Observed values were assessed by MALDI-TOF in the reflector mode unless otherwise noted.
Peptide | HPLC | Mass Spectroscopy | ||||
|---|---|---|---|---|---|---|
| Retention Time | Purity | Calculated | Observed | |||
| min | % | m/z | ||||
| Rat metastin | 20.35 | 99.95 | 5833.91 | 5833.12a | ||
| rKp-10 | 19.35 | 99.07 | 1318.62 | 1318.54 | ||
| hkp-10 | 20.64 | 99.83 | 1302.63 | 1302.80 | ||
| [Ala1]kp-10 | 18.37 | 99.99 | 1226.60 | 1226.52 | ||
| [Ala2]kp-10 | 19.43 | 99.66 | 1275.62 | 1275.65 | ||
| [Ala3]kp-10 | 16.40 | 99.96 | 1203.58 | 1203.63 | ||
| [Ala4]kp-10 | 19.84 | 99.89 | 1275.62 | 1275.54 | ||
| [Ala5]kp-10 | 19.62 | 99.80 | 1302.63 | 1302.54 | ||
| [Ala6]kp-10 | 16.62 | 99.70 | 1242.59 | 1242.68 | ||
| [Ala7]kp-10 | 19.33 | 99.99 | 1332.64 | 1332.79 | ||
| [Ala8]kp-10 | 17.92 | 99.78 | 1276.58 | 1276.38 | ||
| [Ala9]kp-10 | 19.97 | 99.62 | 1233.56 | 1233.25 | ||
| [Ala10]kp-10 | 17.77 | 99.85 | 1226.60 | 1226.43 | ||
↵ a Assessed by MALDI-TOF in the linear mode.