Primer | Nucleotide Sequence (5′ to 3′) |
---|---|
PCR and cloning | |
2B15-54For | AGCCATGGTACCGAGAAATGACAGAAAG |
2B15-101For | AGCCATGGTACCTAGCAGTTATATTTTAAC |
2B15-154For | AGCCATGGTACCTCCTTGGCATGCACCTATTC |
2B15-202For | AGCCATGGTACCCACCAAACACACTAAAA |
2B15-253For | AGCCATGGTACCCTAGGTCATAAAAATTA |
2B15-310For | AGCCATGGTACCGCCACTGTTCTTAACAGT |
2B15-361For | AGCCATGGTACCTACTTCTTCTCTCTATGTC |
2B15-412For | AGCCATGGTACCAAGACATTACTTGCACCACGA |
2B15-458For | AGCCATGGTACCTTGGTGAGTCATATGGC |
2B15-556For | AGCCATGGTACCGAGGCTATTGTATAGAATC |
2B15-595For | AGCCATGGTACCTAGGGTAGGATGGATG |
2B15-705For | AGCCATGGTACCAATCTAGTCCTGCATCC |
2B15-747For | AGCCATGGTACCTTCACAATGCAAGCCTCTG |
2B15-2716For | AAATCTGGGTACCACATAATGCCTGCACT |
2B15-3Rev | AGAACGCGTGGTCTTATGCAATGCTTCTT |
Site-directed mutagenesis/EMSA | |
5′AP-1wt | GTACACACTAATTGGTGAGTCATATGGTATATG |
5′AP-1m | GTACACACTAATTGGCAGAGCATATGGTATATG |
3′AP-1wta* | GGTATATGAATTATGTGTCAACAAAGTTTTAGAAGAC |
3′AP-1ma* | GGTATATGAATTACAGAGCAACAAAGTTTTAGAAGAC |
3′AP-1wtb† | TATGAATTATGTGTCAACAAAGTTT |
3′AP-1mb† | TATGAATTACAGAGCAACAAAGTTT |
3′EREhwt | AAATAAATATGAGGTCATCAATCTTTTGTTG |
3′EREhm | AAATAAATATGAAAAAATCAATCTTTTGTTG |
5′EREhwt | CTTACATATTCTAGGTCATAAAAATTATTG |
5′EREhm | CTTACATATTCTATTTTTTAAAAATTATTG |
EREfwt | CTCTCTATGTCAAGGGCACCGAACAGGC |
EREfm | CTTCTTCTCTCTAAAAAGAGGGCACCGAAC |
X. laevis VitellogeninA2 vitERE | AAAGTCAGGTCACAGTGACCTGATCAAAG |
Quantitative real-time PCR | |
c-Fos-For | TGATGACCTGGGCTTCCCAG |
c-Fos-Rev | CAAAGGGCTCGGTCTTCAGC |
c-Jun-For | CTTCAACCCAGGCGCGCTGAGCA |
c-Jun-Rev | GTCTGAGGCTCCTCCTTCAGGGCCT |
ERα-For | TTCGGCTCCAACGGCCTGGGGGGTTT |
ERα-Rev | GGTACTGGCCAATCTTTCTCTGCCACCCT |
Fra-2-For | TAGATATGCCTGGCTCAGGCAGTGCA |
Fra-2-Rev | TCTTGATCACGCCAGGTCTTGGGA |
18S rRNA-For | CGATGCTCTTAGCTGAGTGT |
18S rRNA-Rev | GGTCCAAGAATTTCACCTCT |
1A3-For | ATGGCAATGTTGAACAATATG |
1A3-Rev | GGTCTGAATTGGTTGTTAGTAATC |
1A7-For | TGGCTCGTGCAGGGTGGACTG |
1A7-Rev | TTCGCAATGGTGCCGTCCAGC |
1A8-For | CTGCTGACCTGTGGCTTTGCT |
1A8-Rev | CCATTGAGCATCGGCGAAAT |
2B28-For | TCTTTTTGATCCCAATGACGCATT |
β-Actin-For | CTGGCGGCACCACCATGTACCCT |
β-Actin-Rev | GGAGGGGCCGACTCGTCATACT |
ChIP assay | |
2B15-ChipF1 | TGCCGTTTGAGTTGTATAATTACTTCTTC |
2B15-ChipR1 | GAATAGGTGCATGCCAAGGAGACC |
2B15-ChipF2 | GGGGGGTTAGAGGCTATTGTATAGA |
2B15-ChipR2 | GAATATGTAAGTAACCTGTCTTATGT |
2B15-ChipR3 | TTAATATCGTGGTGCAAGTAATGTCTTC |
2B15-ChipF3 | GAGTGGGGGGTTAGAGGCTATTGTATAG |
HCG3-ChipF | ATGGTGGACTACTACGAGGT |
HCG3-ChipR | CACCTGCTTGAATCTCCTCT |
TFF1/pS2-For | GGCCATCTCTCACTATGAATCACTTCTGC |
TFF1/ps2-Rev | GGCAGGCTCTGTTTGCTTAAAGAGCG |