Comparison of KD determined by [3H]IP3 binding and FP assays
The KD for each ligand was determined in CLM at 4°C either by competition binding with [3H]IP3 or by FP. Results are means ± S.E.M., n = 3.
| IBC | NT | |||
|---|---|---|---|---|
| [3H]IP3 | FP | [3H]IP3 | FP | |
| nM | ||||
| FITC-IP3 | 2.0 ± 0.2 | 3.0 ± 0.1 | 11.8 ± 0.2 | 12.5 ± 0.6 |
| IP3 | 8.7 ± 1.8 | 9.2 ± 0.8 | 65.2 ± 8.2 | 94.8 ± 7.1 |
| Adenophostin A | 0.7 ± 0.1 | 0.9 ± 0.1 | 6.2 ± 1.6 | 8.6 ± 0.2 |