α6* AChR concatamers, both pentameric and mixtures of free and linked subunits
| AChR Subtype | Constructs (abbreviation) | EC50 | |
|---|---|---|---|
| ACh | Nicotine | ||
| μM | |||
| (α6β2)2β3 | β3(AGS)12α6(AGS)12β2(AGS)6α6(AGS)12β2 | 1.21 ± 0.09 | 0.387 ± 0.051 |
| β3-α6-β2-α6-β2 | n = 6 | n = 5 | |
| β3(AGS)12α6 + α6 + β2 | Expression level too low for accurate EC50 determination | ||
| β3-α6 +α6 + β2 | |||
| (α6β2)(α4β2)β3 | β3(AGS)12α6(AGS)12β2(AGS)6α4(AGS)12β2 | 0.828 ± 0.047 | 0.170 ± 0.079 |
| β3-α6-β2-α4-β2 | n = 5 | n = 4 | |
| β3(AGS)12α6(AGS)12β2 + α4(AGS)12β2 | 0.458 ± 0.046 | 0.306 ± 0.042 | |
| β3-α6-β2 + α4-β2 | n = 4 | n = 5 | |
| (α4β2)(α6β2)β3 | β3(AGS)12α4(AGS)12β2(AGS)6α6(AGS)12β2 | 1.76 ± 0.25 | 0.397 ± 0.122 |
| β3-α4-β2-α6-β2 | n = 5 | n = 4 | |
| β3(AGS)12α4(AGS)12β2 + α6 + β2 | 1.11 ± 0.09 | 0.397 ± 0.070 | |
| β3-α4-β2 + α6 + β2 | n = 4 | n = 7 | |
| β3(AGS)12α4 + α6 + β2 | 1.69 ± 0.14 | 0.584 ± 0.149 | |
| β3-α4 + α6 + β2 | n = 6 | n = 8 | |
| (α6β2)(α4β2)β3 | β3(AGS)6α6(AGS)6β2 + α4(AGS)6β2 | 113 ± 23 | 18.0 ± 4.6 |
| These concatamers with short linkers are not used elsewhere in this paper | n = 6 | n = 4 | |