TABLE 2

Calculation of bias factors for phenethylamine and imidazoline agonists at the α_1A-AR

LogTR is the log of the transduction ratio (τ/K_A), and logTR_n values are normalized to the logTR for norepinephrine. Bias factors in bold are significantly different from 1.0. To avoid propagation of error as a result of multiple data manipulation steps, Student's t tests were carried out on the raw logTR data rather than data normalized to norepinephrine (see Supplemental Table 1).

Agonist	Ca²⁺ Release (n = 6)		cAMP (n = 5)		ECAR (n = 4)		logTR_n [pathway 1-pathway 2]
	Ca²⁺ Release (n = 6)		cAMP (n = 5)		ECAR (n = 4)		[cAMP-Ca²⁺] (Bias Factor)	[ECAR-cAMP] (Bias Factor)	[ECAR-Ca²⁺] (Bias Factor)
	logTR	logTR_n	logTR	logTR_n	logTR	logTR_n	[cAMP-Ca²⁺] (Bias Factor)	[ECAR-cAMP] (Bias Factor)	[ECAR-Ca²⁺] (Bias Factor)
Norepinephrine	8.46 ± 0.14	0.00 ± 0.19	5.48 ± 0.11	0.00 ± 0.16	7.48 ± 0.11	0.00 ± 0.18	0.00 ± 0.25 (1.0)	0.00 ± 0.24 (1.0)	0.00 ± 0.26 (1.0)
Methoxamine	7.24 ± 0.14	−1.22 ± 0.20	4.27 ± 0.13	−1.21 ± 0.18	6.87 ± 0.11	−0.61 ± 0.18	0.01 ± 0.26 (1.0)	0.60 ± 0.25^* (4.0)	0.61 ± 0.26 (4.0)
Phenylephrine	7.94 ± 0.14	−0.52 ± 0.20	4.76 ± 0.14	−0.72 ± 0.18	8.27 ± 0.12	0.79 ± 0.18	−0.20 ± 0.27 (0.63)	1.52 ± 0.26^*** (33)	1.31 ± 0.27^** (21)
Oxymetazoline	8.52 ± 0.15	0.06 ± 0.21	N.A.	N.A.	8.46 ± 0.12	0.98 ± 0.19	N.A.	N.A.	0.91 ± 0.28^* (8.2)
Cirazoline	8.57 ± 0.14	0.11 ± 0.20	6.46 ± 0.22	0.98 ± 0.25	8.10 ± 0.11	0.62 ± 0.18	0.87 ± 0.32^* (7.4)	−0.36 ± 0.31 (0.44)	0.51 ± 0.27 (3.2)
A61603	9.58 ± 0.15	1.12 ± 0.20	7.54 ± 0.12	2.06 ± 0.16	9.03 ± 0.11	1.55 ± 0.19	0.94 ± 0.26^** (8.6)	−0.51 ± 0.25 (0.31)	0.43 ± 0.28 (2.7)

↵* P < 0.05.
↵** P < 0.01.
↵*** P < 0.001.
N.A., not applicable.