β-Alanine dose-response data for GlyRs with unnatural amino acids incorporated at positions 63, 159, and 207
Data are presented as mean ± S.E.M.
| EC50 | nH | R(β-Ala/Gly) | n | |
|---|---|---|---|---|
| μM | ||||
| 63-Phe (WT) | 315 ± 16 | 1.6 ± 0.1 | 1.0 ± 0.3 | 6 |
| 63-F-Phe | 123 ± 4* | 2.2 ± 0.1 | 1.1 ± 0.4 | 5 |
| 63-F2-Phe | 3890 ± 150* | 1.6 ± 0.1 | 0.5 ± 0.2 | 4 |
| 63-F3-Phe | 1590 ± 210* | 1.6 ± 0.4 | 0.2 ± 0.1 | 4 |
| 159-Phe (WT) | 373 ± 8 | 2.0 ± 0.1 | 0.8 ± 0.1 | 4 |
| 159-F-Phe | 470 ± 33* | 1.9 ± 0.2 | 0.9 ± 0.3 | 5 |
| 159-F2-Phe | 3290 ± 120* | 1.7 ± 0.1 | 0.7 ± 0.2 | 6 |
| 159-CN-Phe | 11,500 ± 500* | 1.6 ± 0.1 | 0.7 ± 0.1 | 7 |
| 159-F3-Phe | 20,600 ± 2100* | 1.3 ± 0.2 | 0.1 ± 0.01 | 5 |
| 207-Phe (WT) | 559 ± 30 | 1.7 ± 0.2 | 0.8 ± 0.1 | 3 |
| 207-F-Phe | 615 ± 54 | 1.6 ± 0.2 | 0.5 ± 0.1 | 5 |
| 207-F2-Phe | 32,300 ± 1500* | 2.1 ± 0.2 | 0.1 ± 0.03 | 4 |
| 207-CN-Phe | 5080 ± 140* | 1.6 ± 0.1 | 0.4 ± 0.02 | 7 |
| 207-F3-Phe | 47,900 ± 2100* | 1.9 ± 0.4 | 0.1 ± 0.03 | 4 |
↵* P < 0.05, significantly different from WT for EC50 values (Student's t test).