TABLE 7

Differential modulation of agonist binding at the human GLP-1R Met¹⁴⁹ receptor variant by compound 2

Data were analyzed using a three-parameter logistic equation as defined in eq. 1. pIC₅₀ values represent the negative logarithm of the concentration of agonist that inhibits binding of half the total concentration of radiolabeled antagonist, ¹²⁵I-exendin(9–39). All data are normalized to the maximum ¹²⁵I-exendin(9–39) binding in each individual data set, with non-specific binding measured in the presence of 1 μM exendin(9–39). All values are mean ± S.E.M. of three to four independent experiments, conducted in duplicate.