Effect of ectopic Aurora-A on cell division and mitotic events of curcumin-treated T24 cells
T24 cells transfected with wild-type Aurora-A (pCMV2-flag-Aurora-A) were treated with curcumin for 48 h. The percentages of mitotic cells and mitotic phenotype were scored on the basis of Aurora-A fluorescence and DNA morphology by immunofluorescent microscopy analysis (the number of cells for each condition was larger than 300). Results are representative of three independent experiments.
| Control | 30 μM Curcumin | pCMV2-flag-Aurora-A + 30 μM Curcumin | |
|---|---|---|---|
| % | |||
| Anti-Aurora-A dividing cells | 7.2 | 3.6 | 4.0 |
| Multiple centrosomes | 8.0 | 8.3 | 12.5 |
| Monopolar spindle | 4.0 | 50.0 | 18.7 |