TABLE 1

RGS2 mRNA expression in vascular smooth muscle cells

Endogenously expressed RGS2 mRNA levels were determined by using real-time PCR assays. Only overnight treatment with 10 μM MG-132 had significant effects on RGS2 mRNA expression. Fold values are expressed relative to control values.

TreatmentRGS2 mRNA Level
3 hOvernight
fold
100 nM Digoxin1.31 ± 0.71
100 nM Ouabain0.91 ± 0.17
10 μM Lactacystin0.80 ± 0.061.94 ± 1.92
10 μM MG-1320.56 ± 0.1510.07 ± 5.12***
  • *** p < 0.001, using one-way analysis of variance with Bonferroni's post hoc test for multiple comparisons.