RGS2 mRNA expression in vascular smooth muscle cells
Endogenously expressed RGS2 mRNA levels were determined by using real-time PCR assays. Only overnight treatment with 10 μM MG-132 had significant effects on RGS2 mRNA expression. Fold values are expressed relative to control values.
Treatment | RGS2 mRNA Level | |
---|---|---|
3 h | Overnight | |
fold | ||
100 nM Digoxin | 1.31 ± 0.71 | |
100 nM Ouabain | 0.91 ± 0.17 | |
10 μM Lactacystin | 0.80 ± 0.06 | 1.94 ± 1.92 |
10 μM MG-132 | 0.56 ± 0.15 | 10.07 ± 5.12*** |
↵*** p < 0.001, using one-way analysis of variance with Bonferroni's post hoc test for multiple comparisons.