TABLE 1

Effect of Org 27569 and PSNCBAM-1 on CP55940- and WIN55212-2-stimulated [35S]GTPγS binding in mouse brain membranes

Data are mean ±S.E.M. or with 95% CI.

AgonistVehicle/ModulatorpEC50aEmaxb
(95% CI)% Inhibition
±SEM
%
CP55940DMSO8.2 ± 0.162.6 (57–69)
Org 27569 (10 nM)7.9 ± 0.343.5 (31–56)33.9 ± 6.7
Org 27569 (100 nM)8.6 ± 0.417.98 (12–24)71.8 ± 7.0
Org 27569 (1 μM)95.3 ± 6.6
WIN55212DMSO7.8 ± 0.0998.9 (90–107)
Org 27569 (10 nM)7.7 ± 0.283.1 (68–98)13.9 ± 8.4
Org 27569 (100 nM)8.0 ± 0.290.0 (75–105)8.7 ± 13.3
Org 27569 (1 μM)8.4 ± 0.335.9 (27–45)63.6 ± 4.2
AnandamideDMSO6.7 ± 0.161.4 (55–68)
Org 27569 (10 nM)6.7 ± 0.456.6 (33–80)1.1 ± 12.5
Org 27569 (100 nM)6.4 ± 0.239.0 (30–48)38.1 ± 3.8
Org 27569 (1 μM)7.6 ± 0.317.2 (13–22)71.9 ± 3.4
CP55940DMSO7.0 ± 0.253.7 (47–60)
PSN (10 nM)6.5 ± 0.248.7 (39–58)7.1 ± 12.7
PSN (100 nM)6.6 ± 0.432.6 (24–41)41.8 ± 9.6
PSN (1 μM)6.0 ± 0.515.6 (6–25)72.8 ± 8.4
WIN55212DMSO7.8 ± 0.175.9 (68–84)
PSN (10 nM)7.2 ± 0.378.4 (55–102)0.4 ± 15.0
PSN (100 nM)7.1 ± 0.166.3 (58–74)11.0 ± 3.4
PSN (1 μM)6.8 ± 0.425.9 (13–39)65.0 ± 10.7
  • a Negative logarithm of the agonist EC50 value, determined using nonlinear regression analysis. Values represent the mean ± standard error of the mean (S.E.M.) of four to six experiments.

  • b Maximal agonist effect, determined using nonlinear regression analysis. Values represent the mean with 95% CI of four to six experiments.

  • Significantly different (nonoverlapping confidence limits) from the DMSO vehicle control.