TABLE 6

Effect of Gpp(NH)p and PSNCBAM-1 on [3H]CP55940 dissociation from hCB1 cell membranes

Control data are mean ±S.E.M. of three experiments. Data in the presence of Gpp(NH)p are mean with 95% CI of two independent experiments.

Slow PhaseKoff (Slow)Koff (Fast)
Control57.4 (54.6–59.8)1.1 (0.7–1.7)32 (8.9–111.5)
100 μM Gpp(NH)p29 (25–33)†2.2 (2.4–2.1)†44.3 (44.2–44.4)
100 μM Gpp(NH)p + 2 μM PSN58 (57–59)0.9 (0.8–1.0)49.6 (47.1–52.2)

  • ↵† Significantly different (nonoverlapping confidence limits) from the DMSO vehicle.