TABLE 4

Effect of allosteric modulators on saturation binding of [3H]CP55940 in mouse brain membranes and hCB1 cell membranes

Data shown are mean with 95% CI of three to six independent experiments.

KdBmax
(95% CI)(95% CI)
nMpmol/mg
Mouse Brain Membranes
[3H]CP55 + Vehicle2.67 (1.5–3.8)1.59 (1.3–1.8)
[3H]CP55 + Org (1 μM)2.16 (1.5–2.8)2.43 (2.2–2.7)
[3H]CP55 + Org (10 μM)2.76 (1.7–3.8)3.25 (2.8–3.7)
[3H]CP55 + PSN (1 μM)6.00 (3.1–8.9)3.64 (2.7–4.5)
[3H]CP55 + PSN (10 μM)5.66 (3.6–7.7)2.98 (2.4–3.5)
hCB1
[3H]CP55 + Vehicle0.45 (0.26–0.63)6.4 (5.5–8.4)
[3H]CP55 + PSN (1 μM)0.56 (0.45–0.66)11 (10.8–12.2)
  • Indicates the values are significantly different from vehicle as indicated by non-overlapping confidence limits.