Effect of PSNCBAM-1 on [3H]CP55940 (0.5 nM) dissociation from hCB1 cell membranes
Data shown are mean ±S.E.M. of 3 independent experiments. *P < 0.05 **P < 0.01 one-way ANOVA followed by Dunnett’s multiple comparison.
| Slow Phase | Koff (Slow) | Koff (Fast) | |
|---|---|---|---|
| % | min–1 ×10−2 | min–1 × 10−2 | |
| [3H]CP55 +Vehicle | 59.7 ± 1.9 | 1.1 ± 0.2 | 32 ± 20 |
| [3H]CP55 + 30 nM PSN | 64.4 ± 3.9 | 1.2 ± 0.1 | 63 ± 22 |
| [3H]CP55 + 100 nM PSN | 79.0 ± 4.0** | 0.9 ± 0.1 | 34 ± 9 |
| [3H]CP55 + 2 μM PSN | 100 ± 0** | 0.4 ± 0.04* | — |