TABLE 1

Mutant hEAG1 channels with altered response to ICA

Mutation in hEAG1Mean IC50 for ICA ± S.E.M.nFold increase or decrease (↓) in IC50
µM
(WT)0.44 ± 0.035
V356A**0.08 ± 0.00935.5
V356E**28.8 ± 2.6365
F359A*29.6 ± 9.0367
F359L>304>68
L427A**0.17 ± 0.0132.6
M431A*1.9 ± 0.3454.3
L434A**8.5 ± 0.08519
L434C**4.8 ± 0.2311
M457A>304>68
M458A**20.2 ± 1.2b446
M458Ea0.45 ± 0.083no change
L462A**0.05 ± 0.00538.6
L463A**41.2 ± 8.2b394
Y464A**0.07 ± 0.01436.3
I467A**1.1 ± 0.0532.5
I467E**24.8 ± 4.1b356
F468A**3.1 ± 0.4847
  • hEAG1, human ether-a-go-go type 1; ICA, -nitro-N-(4-phenoxyphenyl)-benzamide (ICA-105574); WT, wild-type.

  • a NS, nonsignificant (P > 0.05).

  • b Effective IC50.

  • * P < 0.01.

  • ** P < 0.001 compared with WT.