TABLE 1

Primer pairs for real-time quantitative polymerase chain reaction

Forward and reverse primers for each gene are listed and were designed to amplify all variants where multiple forms exist. Common gene names are shown and official HUGO (Human Genome Organisation) gene symbols, if different, are given in brackets.

GeneOligonucleotideAccession Number(s)
p57kip2 (CDKN1C)NM_000076.2, NM_001122630.1, NM_001122631.1
 Forward5′-CGG CGA TCA AGA AGC TGT C-3
 Reverse5′-GGC TCT AAA TTG GCT CAC CG-3
GILZ (TSC22D3)NM_001015881.1, NM_198057.2, NM_004089.3
 Forward5′-GGC CAT AGA CAA CAA GAT CG-3
 Reverse5′-ACT TAC ACC GCA GAA CCA CCA-3
RGS2NM_002923
 Forward5′-CCT CAA AAG CAA GGA AAA TAT ATA CTG A-3
 Reverse5′-AGT TGT AAA GCA GCC ACT TGT AGC T-3
CD200NM_005944.5, NM_001004196.2
 Forward5′-GGA CTG TGA CCG ACT TTA AGC AA-3
 Reverse5′-AGC AAT AGC GGA ACT GAA AAC C-3
CRISPLD2NM_031476.3
 Forward5′-CAA ACC TTC CAG CTC ATT CAT G-3
 Reverse5′-GGT CGT GTA GCA GTC CAA ATC C-3
GAPDHNM_002046.4, NM_001256799.1
 Forward5′-TTC ACC ACC ATG GAG AAG GC-3
 Reverse5′-AGG AGG CAT TGC TGA TGA TCT-3
  • GAPDH, glyceraldehyde 3-phosphate dehydrogenase.