TABLE 1

Equilibrium binding parameters of 125I-CCL2, [3H]INCB3344, and [3H]CCR2-RA, determined on U2OS membranes expressing CCR2

Data are presented as mean ± S.E.M. of three experiments performed in duplicate.

125I-CCL2[3H]INCB3344[3H]CCR2-RA
KD (nM)0.068 ± 0.014a0.90 ± 0.03b5.8 ± 0.2c
Bmax (pmol/mg)0.31 ± 0.03a7.1 ± 0.2b9.7 ± 0.2c
  • a Homologous displacement of 125I-CCL2 from CCR2 at 37°C.

  • b Saturation binding of 0.5–45 nM [3H]INCB3344 to CCR2 at 25°C.

  • c Saturation binding of 0.2–75 nM [3H]CCR2-RA to CCR2 at 25°C.