G protein activation by CCL2 measured by [35S]GTPγS binding
Potency and maximum effect of CCL2 in the absence or presence of different concentrations of CCR2-RA-[R]. Data are presented as mean ± S.E.M. of at least three experiments
| pEC50 ± S.E.M. | Emax ± S.E.M. (%) | |
|---|---|---|
| Control | 8.3 ± 0.1 | 104 ± 4 |
| +1 nM INCB3344 | 7.6 ± 0.1* | 103 ± 7 |
| +3 nM INCB3344 | 7.0 ± 0.3* | 102 ± 14 |
| +10 nM INCB3344 | 6.2 ± 0.9 | ND |
| +10 nM CCR2-RA-[R] | 7.7 ± 0.1* | 81 ± 6** |
| +30 nM CCR2-RA-[R] | 7.7 ± 0.1* | 55 ± 4*** |
| +100 nM CCR2-RA-[R] | 7.6 ± 0.1* | 30 ± 3*** |
ND, not determined.
↵* P < 0.05; **P < 0.001; ***P < 0.0001 versus control, Student’s t test.