TABLE 2

Affinity and potency values for dynorphin peptides using GloSensor and Tango assays

PeptidesG Protein EC50G Protein EmaxArrestin EC50Arrestin EmaxBias Factor
nMnM
Salvinorin A5.183 (−8.29 ± 0.10)99.75.75 (−8.24 ± 0.06)97.241
Dynorphin A8.12 (−8.09 ± 0.07)101268 (−6.57 ± 0.11)74.834 G protein
Dyn 1–857.7 (−7.24 ± 0.05)106720 (−6.14 ± 0.11)89.94 G protein
Dyn 1–910.2 (−7.99 ± 0.06)101600 (−6.22 ± 0.09)64.716 G protein
Dyn 1–113.26 (−8.49 ± 0.08)101450 (−6.35 ± 0.09)75.844 G protein
Dyn 1–132.07 (−8.68 ± 0.07)96.697.8 (−7.01 ± 0.07)72.434 G protein