TABLE 3

K_i values from ³H-dopamine uptake inhibition experiments in wild-type hDAT and hDAT mutations of residues in the central binding site diverging between hSERT and hDAT

Confidence levels are provided in Supplemental Table 3.

hDAT Variant	Mazindol	Analog
hDAT Variant	Mazindol	1	2	3	4
	nM
Wild type	140	92	17.1	11.5	68
F76Y	1700	260	52	71	177
S149A	196	210	21	13.4	71
V152I	80	37	5.4	6.5	33
G153A	340	76	12.7	7.7	94
F155Y	61	59	96	5.6	14.7
A423T	69	16.8	5.1	5.4	14.0
M427L	290	101	21	13.4	88
A480T	82	13.4	7.7	5.7	8.1
F76Y_V152I	5700	1400	230	230	1200
F76Y_M427L	4100	550	76	127	450
V152I_M427L	159	68	10.6	7.5	44
F76Y_V152I_M427L	17,400	1660	163	204	2300