TABLE 1

The effect of extracellular αKG (5 μM) on the kinetics of OAT1-mediated PAH and cidofovir transport

Data are mean ± S.D. of four experiments. Significant differences in IC₅₀ values (between control and +5 μM αKG) were determined by unpaired Student’s t test.

Substrate	Treatment	J_max	K_m	T_eff
		pmol × mg protein⁻¹ × min⁻¹	μM	μl × mg protein⁻¹ × min⁻¹
PAH	Control	480 ± 117	12.7 ± 5.4	41.0 ± 13
PAH	+5 μM αKG	585 ± 159	28.1 ± 6.8^*	21.4 ± 7.2^*
Cidofovir	Control	572 ± 384	51.3 ± 13	11.0 ± 6.9
Cidofovir	+5 μM αKG	518 ± 300	125 ± 38**	4.35 ± 2.7^*

↵* P < 0.05; **P < 0.01.