TABLE 1

Substrates of human and mouse UGT8 expressed in HEK293T cells

UDP glycosyltransferase 8 (UGT8) activities were assayed as in Materials and Methods with 0.2 mM of the listed substrate and 0.5 or 1 mM UDP-galactose. Activities are given as picomoles per minute per milligram lysate protein from a representative experiment.

SubstrateHuman UGT8 ActivityMouse Ugt8 Activity
pmol/min per mg
α-Hydroxy fatty acid ceramide226
N-Hexanoyl-d-sphingosine6N.D.
Cholic acid129376
Chenodeoxycholic acid73389
Deoxycholic acid317439
Lithocholic acid49113
Hyodeoxycholic acid1992
Ursodeoxycholic acid352
Chrysin2ND
  • N.D., not done.