TABLE 3

Activation of G_q, PKC-ERK, and EGFR-ERK by AT₁ receptor ligands

HEK293 cells stably expressing the AT₁ receptor were assayed as described in Materials and Methods. EC₅₀ and E_max are expressed as the means ± S.D. of values obtained in at least three independent experiments performed in duplicate.

	G_q		PKC-ERK		EGFR-ERK
	EC₅₀	E_max	EC₅₀	E_max	EC₅₀	E_max
	nM	% AngII	nM	% AngII	nM	% AngII
AngII	2.4 ± 1.6	100	1.2 ± 0.1	100	2.8 ± 0.5	100
[Sar¹]AngII	3.3 ± 0.8	98 ± 8	3.0 ± 0.9	92 ± 4	4.2 ± 1.9	94 ± 2
[Ile⁴]AngII	3104 ± 106	67 ± 2	523 ± 93	80 ± 10	1327 ± 264	93 ± 8
[Sar¹Ile⁴]AngII	686 ± 52	43 ± 7	32 ± 5	94 ± 7	50 ± 9	95 ± 5
[Ile⁸]AngII	NR	NR	55 ± 13	38 ± 4	40 ± 14	27 ± 3
[Sar¹Ile⁸]AngII	NR	NR	45 ± 14	28 ± 4	11 ± 3	27 ± 1
AngIII	51 ± 19	87 ± 8	4.0 ± 0.6	88 ± 3	9.2 ± 1.9	88 ± 3
[Ile⁸]AngIII	NR	NR	40 ± 5	25 ± 3	21 ± 4	30 ± 4

NR, no response.